2016 年第 4 期 第 14 卷

血浆微泡乳腺癌耐药蛋白mRNA水平与乳腺癌化疗疗效及乳腺癌组织相关基因的关系

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关键词：乳腺癌化疗微泡乳腺癌耐药蛋白增殖凋亡

• 摘要：
• 【摘要】摘 要：目的 探讨血浆微泡乳腺癌耐药蛋白mRNA水平与乳腺癌化疗疗效及乳腺癌组织相关基因的关系。方法 选择接受新辅助化疗的乳腺癌患者60例作为乳腺癌组，选择同时期健康体检者60例作为对照组。检测血浆微泡乳腺癌耐药蛋白的mRNA水平以及乳腺癌组织相关基因中凋亡基因caspase-3、caspase-9和增殖相关基因Survivin、Bcl-2的mRNA含量。结果 乳腺癌组患者血浆微泡中BCRP的mRNA含量为（254.56±34.52）显著高于对照组（100±16.62），差异有统计学意义（t=31.289, P<0.05）；不同化疗效果患者血浆微泡中BCRP的mRNA含量有差异，CR/ PR组患者血浆微泡中BCRP的mRNA含量显著低于SD+PD组，差异均具有统计学意义(P<0.05)；且乳腺癌组织中凋亡基因caspase-3、caspase-9的mRNA含量低于癌旁正常组织，增殖相关基因Survivin、Bcl-2的mRNA含量高于癌旁正常组织，差异有统计学意义（P<0.05），血浆微泡中BCRP的mRNA含量与caspase-3、caspase-9的mRNA含量呈负相关，与Survivin、Bcl-2的mRNA含量呈正相关.结论 血浆微泡中BCRP的mRNA含量可作为乳腺癌辅助化疗疗效的预测指标，为乳腺癌化疗提供指导依据。 关键词：乳腺癌；化疗；微泡；乳腺癌耐药蛋白；增殖；凋亡
• 【abstract】objective To investigate the relationship between plasma microvesicles breast cancer resistance protein and mRNA levels of chemotherapy in breast cancer and breast cancer associated genes in tissues.Methods Select breast cancer patients receiving neoadjuvant chemotherapy in 60 cases of breast cancer group, choose the same period, 60 cases of healthy people as a control group.Plasma microbubbles breast cancer resistance protein mRNA levels and mRNA levels of genes associated with breast cancer and apoptosis gene caspase-3, caspase-9 and proliferation related gene Survivin, Bcl-2's.Results Plasma in patients with breast cancer group microbubbles of BCRP mRNA content was (254.56 ± 34.52) was significantly higher (100 ± 16.62), the difference was statistically significant (t = 31.289, P <0.05);The effect of chemotherapy in patients with different plasma microvesicles of BCRP mRNA levels are different, the plasma of patients CR / PR group microvesicles of BCRP mRNA content was significantly lower than SD + PD group, the differences were statistically significant (P <0.05);And apoptosis in breast cancer gene caspase-3, mRNA content of caspase-9 is lower than the adjacent normal tissue proliferation related gene Survivin, mRNA content of Bcl-2 is higher than the adjacent normal tissues, the difference was statistically significant (P <0.05),Plasma microvesicles BCRP mRNA content and caspase-3, mRNA content of caspase-9 was negatively correlated positively correlated with Survivin, mRNA content of Bcl-2 was.Conclusion Plasma microvesicles of BCRP mRNA levels can be used as adjuvant chemotherapy in breast cancer predictor to provide guidance based chemotherapy for breast cancer.