2016 年第 4 期 第 14 卷

RNAi沉默肺癌A549细胞中CXCR6基因对其细胞增殖、体外侵袭力和裸鼠成瘤能力的影响

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关键词：RNA干扰CXCR6基因肺癌增殖侵袭力

• 摘要：
• 【摘要】目的：应用RNA干扰技术（RNAi）沉默肺癌A549细胞中CXCR6基因的表达，探讨其对A549细胞增殖、体外侵袭力和裸鼠成瘤能力的影响。方法：MTT法和Transwell小室模型检测稳定沉默CXCR6基因的A549细胞增殖和细胞侵袭迁移能力。将稳定沉默CXCR6的A549细胞与对照细胞皮下接种裸鼠，观察接种后肿瘤生长情况。结果：稳定沉默CXCR6基因的A549细胞与正常A549细胞相比，前者增殖速率明显下降(P<0.01)，增殖平均抑制率为30.70%；前者的体外侵袭力也明显降低(P<0.01)，侵袭平均抑制率为74.93%，迁移平均抑制率为70.19%。裸鼠体内实验显示，稳定沉默CXCR6基因的A549细胞与正常A549细胞相比，其裸鼠体内成瘤能力降低(P<0.05)，瘤体重量下降了62.5%。结论：沉默CXCR6基因的表达，从而抑制A549细胞的增殖、体外侵袭力和抑制成瘤能力，CXCR6在肺癌的发生发展中发挥着重要作用。
• Objective To study the effect of CXCR6 gene on proliferation, cell invasiveness and tumorigenicity of lung cancer A549 cells by silencing CXCR6 gene using RNA interference technique.Methods Proliferation, cell invasiveness and metastasis distribution of A549 cells were detected by MTT assay and Transwell assay.Meanwhile, the growth of subcutaneously implanted tumors was observed in nude mice after inoculated with A549cells with CXCR6 stably silenced and the control A549 cells.Results Proliferation of CXCR6 silenced A549 cells was significantly suppressed (P<0.01), proliferation inhibition rate on average is about 30.70%, and Compared with the shRNA-control and blank-control cells,the cell invasiveness and metastasis of CXCR6-shRNA cells were reduced by 74.93% and 70.19% , respectively. The tumorigenicity of A549 cells in nude mice was inhibited when CXCR6 was silenced(P<0.01), tumor weight was reduced by 62.5%. .Conclusion phU6 GFP Neo CXCR6 plasmid stably transfecting into lung cancer A549 cells can effectively silence CXCR6 gene expression, inhibit cell proliferation, influence invasiveness and metastasis and inhibit tumorigenicity of A549 cells. CXCR6 plays an important role in the development of lung cancer.