2017 年第 2 期 第 15 卷

miR-214、miR-503在上皮性卵巢癌组织及血清外泌小体中表达的研究

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关键词：miR-214miR-503上皮性卵巢癌（EOC）化疗耐药外泌小体

• 摘要：
• 【摘要】目的：检测正常卵巢、化疗敏感上皮性卵巢癌及耐药上皮性卵巢癌组织及血清外泌小体中p53相关miR-214、miR-503的表达差异，初探p53相关miRNA与上皮性卵巢癌（EOC）耐药的相关性。方法：免疫荧光法检测正常卵巢、化疗敏感EOC及耐药EOC组织中p53的表达。RT-PCR法对比血清外泌小体及上述三种组织中p53相关miR-214、miR-503表达差异。结果：免疫荧光发现，与正常组织相比，p53在EOC中荧光较弱，其中化疗耐药EOC与敏感EOC间无显著差异（p＞0.05）。与敏感组相比，耐药组血清外泌小体中miR-214上调3倍，miR-503下调2倍，与组织中变化一致。结论：p53相关miR-214及miR-503由外泌小体携带进入血液循环，并可能在卵EOC化疗耐药的发生发挥重要作用。
• Objective： to exam the expression of p53 related miR-214 and miR-503 in chemo-sensitive and chemo-resistance epithial ovarian caner tissue and serum exosomes. Method: p53 expression level in tissue was examed by ICH. RT-PCR was use to detect the expression of miR-214 and miR-503 in tissues. RT-PCR was used to exam the expression of miR-214 and miR-503 in serum exosomes. Results:there was no significant difference of p53 expression in chemo-resistant and chemo-sensitive EOC, but there was sigenificant difference of miR-214 and miR-503 level in both tissue and serum exosomes in chemo-resistant and chemo-sensitive EOC, Conclusion: p53 related miR-214 and miR-503 might play an important role in chemo-resistance of EOC.