2017 年第 1 期 第 15 卷

黄芪多糖逆转Ec109/DDP食管癌细胞顺铂耐药的可能机制

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关键词：食管癌黄芪多糖顺铂耐药机制

• 摘要：
• 【摘要】目的 研究黄芪多糖逆转EC109/DDP食管癌细胞顺铂耐药的可能机制。方法 将EC109/DDP顺铂耐药食管癌细胞分为CON组（未行任何药物干预）、APS组（仅黄芪多糖干预）、DDP组（仅顺铂干预）及APD组（黄芪多糖及顺铂干预），比较四组细胞吸光度、细胞周期、凋亡率、MRP和GST-π基因的差异。结果 APD组Ec109/DDP食管癌细胞D1-D6吸光度、S期、G2/M期和增殖指数均显著低于CON组、APS组和DDP组细胞（均P<0.05），G0/G1期和凋亡率均显著高于CON组、APS组和DDP组细胞（均P<0.05）。CON组和DDP组EC109/DDP顺铂耐药食管癌细胞MRP和GST-π基因mRNA表达均无明显差异（均P>0.05）；APS组和APD组细胞MRP和GST-π基因mRNA表达均无明显差异（均P>0.05），但两组细胞MRP和GST-π基因mRNA表达均显著低于CON组和DDP组细胞（均P<0.05）。结论 黄芪多糖可逆转Ec109/DDP顺铂耐药食管癌细胞对顺铂的耐药性，其可能机制与黄芪多糖显著降低MRP和GST-π基因表达有关。
• Objective To study the mechanism of Astragalus polysaccharide’s reversing effect on Ec109/DDP esophagus cancer cell’s resistance to Cisplatin. Methods Ec109/DDP esophagus cancer cells were divided into CON group (no astragalus polysaccharide and Cisplatin treatment), APS group (only astragalus polysaccharide treatment), DDP group (only Cisplatin treatment) and APD group (Astragalus polysaccharide and Cisplatin treatment) according to their treatment methods. The differences of cell absorption, cell cycle, apoptosis and expressions of MRP and GST-π were compared. Results The D1-D6 absorption, S stage, G2/M stage and PI in APD group were significantly lower than in CON group, APS group and DDP group (All P<0.05), but G0/G1 stage and cell apoptosis rate were higher (All P<0.05). The differences of MRP and GST-π mRNA expressions were not statistics significant between APS group and APD group, but the were lower than in CON group and DDP group (All P<0.05). Conclusion Astragalus polysaccharide could significantly reverse Ec109/DDP esophagus cancer cell’s resistance to Cisplatin, its mechanism may be related with lowering down MRP and GST-π’s expression.