2017 年第 1 期 第 15 卷

ALA-PDT对食管癌Eca-109凋亡相关基因表达的影响

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关键词：食管癌凋亡相关基因光动力靶基因

• 摘要：
• 【摘要】目的 凋亡相关基因Bak-1、FADD在食管癌Eca109细胞ALA-PDT的作用及初步探讨其在食管癌ALA-PDT凋亡通路的作用。方法 实验分为对照组和ALA-PDT组，治疗组细胞给予ALA（终浓度为0.25mM）孵育6小时后在630 nm Diomed 光动力治疗系统下进行光照（功率200 mW/cm2, 照光150 s，光剂量30J/cm2）。光照后0.5 h,3 h和24 h收集细胞，提取细胞RNA，采用Realtime PCR法检测Bak-1、FADD基因表达的变化。结果 FADD在ALA-PDT作用于Eca-109细胞3 h和24 h后其表达均显著下调(P均＜0.05），Bak-1作用后0.5 h,3 h和24 h其表达相对对照组均下调，仅在作用后3 h有统计学差异，其余与对照组相比其差异无统计学意义（P均＞0.05）。结论 Fas通路可能为ALA-PDT诱导Eca-109细胞凋亡的通路之一，FADD可能为该凋亡通路的靶基因之一。
• Objective To investigate the role of apoptosis related gene Bak-1 and FADD in ALA mediated PDT(ALA-PDT) in esophageal cell line Eca-109 and explore its role in apoptosis pathway in PDT. Methods The experiment was divided into two groups: the control group and PDT group. The cells of PDT group were incubated with ALA to the final concentration of 0.25 mM for 6 h in dark, and irradiated with the light dose of 30 J/cm2 (200 mW/cm2, 150 s) under the 630 nm laser system. Total RNA were extracted from Eca-109 cells at 0.5 h and 3 h and 24 h after ALA-PDT. Then quantitative real-time polymerase chain reaction(QRT-PCR) assay was used to detect the gene expression changes of Bak-1 and FADD. Rerults The expression of FADD was significant down regulation at 3 h and 24 h psot-PDT in the Eca-109 cell, and Bak-1 were down-regulation, at 0.5 h and 3 h and 24 h after treatment, but the difference contrast to the control group was not significant except at 3 h(P＞0.05). Conclusion Fas pathway may play the role in the apoptosis way and FADD may be the target gene in ALA-PDT in Eca-109.